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Encompassing Amino Acids: 2-186(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to KRAS(G12C) Coupled Nucleotide Exchange Assay Kit (BPS Bioscience #78004) without SOS1/GTP mixture. KRAS (G12D), Isoform A, His-Tag, GDP-Loaded Recombinant (BPS Bioscience #101312) and GppNHP loaded KRAS (G12D) (BPS Bioscience #101481) were titrated at various concentrations.
Description: Recombinant human KRAS (KRAS proto-oncogene GTPase), isoform a, encompassing amino acids 2-186(end). This construct contains an N-terminal His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease, BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and loaded with GppNHp, a non-hydrolyzable GTP analog. Unbound GppNHp was removed by spin column. Ready for use in KRAS-RAF binding studies or inhibitor assays.
Format: Aqueous buffer solution
Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl, and 1 mM DTT
Genbank: NM_033360
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution
Tags: N-terminal His-tag
Uniprot: P01116
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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