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Encompassing Amino Acids: 2-185
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Intrinsic GTPase reaction of KRAS (G12V) was conducted at room temperature for 60 min using GTPase-Glo (Promega) reagents with 1 µM GTP.
Description: Human KRAS also known as C-K-RAS, CFC2, and K-RAS2A, GenBank Accession No. NM_033360, a.a. 2-185, with G12V mutation and N-terminal His-tag, and expressed in a Baculovirus infected Sf9 cell expression system. MW=22 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 120 mM Imidazole
Genbank: NM_033360
Storage Stability: At least 6 months at -70°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-Tag
Uniprot: P01116
Biosafety Level: Not applicable (BSL-1)
References: 1. Martinelli, P., et al., Gut. 2016;65:476-486.2. Maraver, A., et al., Cancer Cell. 2012 Aug;22(2):222-234.
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