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Applications: Useful for the study of MBT domain binding assays, screening inhibitors, and selectivity profiling
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The L3MBTL1 TR-FRET Assay Kit is designed to measure theinhibition of L3MBTL1 binding to its substrate in a homogeneous 384 reaction format.This FRET-based assay requires no time-consuming washing steps, making it especiallysuitable for high throughput screening applications. The assay procedure isstraightforward and simple; a sample containing terbium-labeled donor, dye-labeledacceptor, L3MBTL1, substrate, and an inhibitor is incubated for sixty minutes. Then, thefluorescence intensity is measured using a fluorescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed
Uniprot: Q9Y468
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Min, J., et al., Nat Struct Mol Biol.2008 Jan,15(1):114
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