Transfection

Transfection

The Principle of Nucleofector™ Technology:

By applying electrical pulses, small pores are generated short-term in the cell membrane. An interplay of preprogrammed Nucleofection protocols and coordinated reagents result in perfectly adapted conditions for a successful transfection for the particular cell type used. The substrate is not only taken up by the cytoplasm, but also by the nucleus due to the optimized conditions. This process allows high transfection rates with an efficiency of up to 99%.

We offer the following platforms:

  • For maximum flexibility: 4D Nucleofector (X, Y and LV units)
  • For medium-throughput: add-on 96-well shuttle
  • For high throughput: HT Nucleofector
  • The base unit: Nucleofector 2b

The Nucleofector kits contain special transfection cuvettes, Nucleofector reagents (buffer and supplement) and the control plasmid pmaxGFP. The buffer and its supplement are optimized specifically for respective primary cells.

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  1. 4D-Nucleofector™ Y Unit
    4D-Nucleofector™ Y Unit
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    Sku LONAAF-1003Y
    Manufact. SkuAAF-1003Y
    Packaging Unit1 Stück
  2. 4D-Nucleofector™ LV Unit
    4D-Nucleofector LV Unit
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    Sku LONAAF-1002L
    Manufact. SkuAAF-1002L
    Packaging Unit1 Stück
  3. 4D-Nucleofector™ cuvette rack
    4D-Nucleofector Cuvette Rack
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    Sku LONAAK-2002
    Manufact. SkuAAK-2002
    Packaging Unit1 Stück
  4. 4D-Nucleofector™ module rack
    4D-Nucleofector Module Rack
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    Sku LONAAK-2001
    Manufact. SkuAAK-2001
    Packaging Unit1 Stück
  5. 4D-Nucleofector™ LogWare
    The 21 CFR part 11 compliant software for first generation 4D-Nucleofector® Systems running under software version 4
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    Sku LONSAAF-1001
    Manufact. SkuSAAF-1001
    Packaging Unit1 piece
  6. 1 mL Nucleocuvette™ Cartridge Box (2pcs)
    1 mL Nucleocuvette™ Cartridge Box (2pcs)
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    Sku LONV4LN-7002
    Manufact. SkuV4LN-7002
    Packaging Unit1 Piece
  7. LV Nucleocuvette™ Cartridge Box (weld.)
    LV Nucleocuvette™ Cartridge Box (weld.)
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    Sku LONV4LN-7020W
    Manufact. SkuV4LN-7020W
    Packaging Unit1 Piece
  8. LV Nucleocuvette™ Cartridge Box (1 pc)
    LV Nucleocuvette™ Cartridge Box (1 pc)
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    Sku LONV4LN-7020
    Manufact. SkuV4LN-7020
    Packaging Unit1 Piece
  9. 4D-Nucleofector™ LV Reservoir (weld.)
    4D-Nucleofector™ LV Reservoir (weld.)
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    Sku LONV4LR-1001W
    Manufact. SkuV4LR-1001W
    Packaging Unit1 Piece
  10. 96-well Nucleocuvette™ Plate
    96-well Nucleocuvette Plate (via MTA only)
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    Sku LONVHNP-1001
    Manufact. SkuVHNP-1001
    Packaging Unit1 PC
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18 items

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