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Applications: Useful for studying protein binding and screening small molecular inhibitors
Assay Conditions: 5 µl FGL1 was used with a final concentration of 100 nM along with 5 µl of titrated LAG3 [B] catalog #71147 titrated from 150-0 ng/rxn and incubated at room temperature for 1 hour. 10 µl 1:250-fold diluted Ni acceptor beads Perkin Elmer #AL108C were added to the plate and incubated at room temperature for 30 min. 10 µl 1:125-fold diluted Strep Donor Beads Perkin Elmer #67600025 were added and incubated at room temperature for 1 hour.
Description: Human secreted LAG3, Fc fusion protein, also known as Lymphocyte-Activation Gene 3 and CD223, with C-terminal Avi-Tag™. GenBank Accession No. NM_002286, a.a. 23-450 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag™ technology. MW = 74.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Endotoxin Level: <1 EU/µg
Format: Aqueous buffer solution
Formulation: 20 mM NaOAC, pH 5.5, 500 mM NaCl, 0.02% Tween 20 and 10% glycerol
Genbank: NM_002286
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Avi-Tag™
Uniprot: P18627
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Byrnes, et al., Cytometry A. 2012 Sep;81(2):165-8.
2. Di Carlo, et al., J. Pathol. 2005 Jan; 205(1):82-91.
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