Target: LAR
Conjugate: PerCP
Product Type: Monoclonal
Clone Number: N165/38 (Formerly sold as S165-38)
Immunogen: Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.
Swiss-Prot: P10586
Purification: Protein G Purified
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~85kDa (full length protein is 210 kDa – smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.
Cellular Localization: Membrane
Scientific Background: PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule. PTPRF removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function (1,2,3,4). Anti-PTPRF (C-terminal) antibody is specific for the extracellular and cytoplasmic subunits of human PTPRF (approx. 210, 150 and 85 kDa). Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.
References: 1. Streuli M. M., et al. (1992) EMBO Journal, 11(3), 897-907.2. Mooney R.A. and C.M LeVea. (2003) Current Topics in Medicinal Chemistry, 3(7), 809-819.3. Chagnon M.J., et al. (2004) Biochemistry and Cell Biology, 82(6), 664-675.4. Ahmad F.F., and B.J. Goldstein (1997) Journal of Biological Chemistry, 272(1), 448-457.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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