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Encompassing Amino Acids: 1330-1620
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and 160 ng/µl LAR. Assay was done at 30°C for 5 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
Background: PTPRF or LAR is a member of the protein tyrosine phosphatase family with an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains. PTPRF has been shown to function in the regulation of epithelial cell-cell contacts at adherents junctions as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance.
Description: Human transmembrane leukocyte antigen related PTP (LAR), GenBank accession No. NM_130440, a.a. 1330-1620 (catalytic domain) with N-terminal GST-tag, MW=58 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Genbank: NM_130440
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: P10586
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. H. Cho et al. Biochemistry 1991, 30: 6210-6216.
2. H. Cho et al. Biochemistry 1992, 31: 133-138.
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