Formulation: TBS, pH 7.5, with 10% sucrose
Formula Weight: 0
Shelf life (days): 180
Notes: LipidLaunch™ ALC-0315 LNP (Luciferase) is a solution containing lipid nanoparticles (LNPs) composed of the ionizable cationic amino lipid ALC-0315 (Item No. 34337), cholesterol (Item Nos. 9003100) / 39088), the phospholipid 1,2-distearoyl-sn-glycero-3-PC (1,2-DSPC; Item Nos. 15100 / 39189), and the lipid excipient ALC-0159 (Item No. 34336) at a molar ratio of 46.3:42.7:9.4:1.6 and encapsulating mRNA encoding firefly luciferase protein. It is intended for proof-of-concept experiments to determine whether ALC-0315-based LNPs can effectively lead to the expression of a protein of interest in a target cell type, either in vitro or in vivo. Firefly luciferase is an enzyme that catalyzes the oxidation of ATP-dependent D-luciferin, resulting in chemiluminescence at a wavelength of approximately 560 nm. Suggested in vitro use: Thaw LNPs on ice with occasional gentle swirling (do not vortex). Using a gentle pipetting technique, dilute 1:100-1:500 in complete cell culture media (with serum) and add to subconfluent cells in a luminescence-compatible tissue culture plate. Use a luciferase reporter assay substrate and read luminescence on a plate reader. Optimal conditions are highly dependent on cell type.
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