Shelf life (days): 180
Notes: Lipid nanoparticles (LNPs) are a subset of lipid-based drug delivery (LBDD) systems that utilize ionizable lipids, such as SM-102, for the delivery of RNA payloads to cells.{63013,71110} They frequently consist of a lipid shell composed of structural phospholipids, cholesterol, and PEGylated lipids that surround an internal aqueous core, where the ionizable lipids organize into inverted micelles around the encapsulated RNA. Release of LNP cargo into target cells is heavily influenced by the ionizable lipid component, which undergoes protonation in the acidic environment of the endosomes, resulting in membrane disruption and release of cargo into the cell.{71109} SM-102 is an ionizable cationic amino lipid that has been used in combination with other lipids in the formation of LNPs.{61317,66449} Traditional lipid-based transfection methods commonly use fixed cationic lipids, resulting in high toxicity.{63381} Cayman’s LipidLaunch™ Loadable LNP SM-102 Kit’s use of the cationic aminolipid SM-102 facilitates efficient RNA transfection with reduced toxicity compared to traditional methods. Cayman’s LipidLaunch™ Loadable LNP SM-102 Kit provides cargo-ready, empty, lyophilized SM-102 LNPs specially formulated for in vitro RNA transfection. Following resuspension, these LNPs are capable of rapid encapsulation of RNA, without the need for incubation steps, enabling subsequent delivery to target cells. LipidLaunch™ Loadable LNPs demonstrate very low toxicity compared to traditional lipid-based transfection methods. Conventionally loaded LNPs typically require specialized equipment to ensure consistent control of particle size, while also requiring larger reaction volumes. LipidLaunch™ Loadable SM-102 LNPs enable users to encapsulate RNA cargo in reaction volumes as low as 50 µl, offering greater flexibility in the scale of preparation while preserving costly RNA cargo.
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