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Application Note: This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase). Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 66 kDa in size corresponding CE1 by western blotting in the appropriate cell lysate or extract. In western blot analysis endogenous protein present in human tissue or fluids, multiple bands of CE may appear corresponding to variable amount of glycosylation on the molecule.
Concentration Value: 90 mg/mL
ELISA Dilution: 1:5,000 - 1:20,000
Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:2,500 – 1:5,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.
Physical State: Liquid (sterile filtered)
Purity and Specificity: This product was prepared from monospecific antiserum by delipidation and defibrination. The antibody detects recombinant hCE1 in cell lysates and native Liver Carboxylesterase-1 present in human tissue or fluids in various assays (ie. IL-1 stimulated hCE1 production from fibroblasts).
Background: Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.
Low Endotoxin: No
Other: User Optimized
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