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Encompassing Amino Acids: 1-348
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human recombinant LOK1, also known as STK10 (GenBank Accession No. NM_005990), a.a. 1-348 with N-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 43.2 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: NM_005990
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: O94804
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Walter S.A. et al., J. Biol. Chem. 278 (20), 18221-18228 (2003).
2. Tao L.et al., Biochem. J. 363 (PT 1), 175-182 (2002).