Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 158-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µl reaction mix containing assay buffer with 50 mM Tris HCl pH 8.8, 50 mM NaCl, 1mM DTT, 0.01% Tween-20, histone H3 peptide substrate, and LSD1 (6.7 - 40 ng) add to the wells. Incubate for 1 hr. Add antibody against unmethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Description: Human LSD1 [also known as KDM1A (lysine (K)-specific demethylase 1A) or AOF2, amine oxidase (flavin containing) domain 2)] GenBank Accession No. NM_015013, a.a. 158-end with N-terminal GST-tag, MW=103 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_015013
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O60341
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lan,F. et al., Nature 448, 7154, 718-722, 2007.
2. Forneris,F. et al., J. Biol. Chem. 282, 28, 20070-20074, 2007.Application Reference(s):
1.
A scintillation proximity assay for histone demethylases (2014)
2. Crystal structure of the histone lysine specific demethylase LSD1 complexed with tetrahydrofolate (2014)
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