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Applications: Great for studying enzyme kinetics and HTS applications.
Contraindications: Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.
Description: The LSD2(KDM1B) Homogeneous Assay Kit (Monomethyl) is designed to measure the activity of lysine-specific demethylase (LSD2) for screening and profiling applications. LSD2, also called KDM1B, is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3, thereby acting as a corepressor of transcription. LSD2 is a potential target for drug development. The LSD2 Homogeneous Assay Kit (Monomethyl) comes in a convenient AlphaLISA® format, with biotinylated, mono-methylated histone H3 (1MeK4) peptide substrate, primary antibody to detect non-methylated product, LSD2 assay buffer, and purified LSD2 for 384 enzyme reactions. The key to the LSD2 Homogeneous Assay Kit (Monomethyl) is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase detection. First, a sample containing LSD2 enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Storage Stability: At least one year from date of receipt when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The LSD2 Homogeneous Assay Kit 1 comes in a convenient AlphaLISA® format, with biotinylated, mono-methylated histone H3 (1MeK4) peptide substrate, primary antibody to detect non-methylated product, LSD2 assay buffer, and purified LSD2.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1 Binda, C., et al., J. Am. Chem. Soc. 2010; 132(19): 6827-6833.2. Welte, M., et al., Cell Press. 2005; 15(14): 1266-1275.
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