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Applications: This kit is useful for screening for inhibitors of LIGHT binding to LTβR.
Background: Lymphotoxin β receptor (LTβR) and herpes virus entry mediator (HVEM) are the physiological receptors of LIGHT (tumor necrosis factor superfamily member 14 (TNFSF14) or CD258). The activation of the LTβR/HVEM:LIGHT co-signaling pathways induces the production of pro-inflammatory molecules, like NF-κB, chemokines and adhesion molecules. LTβR is up regulated in hepatitis and hepatocellular carcinoma, and influences lipid metabolism and atherosclerosis. It also regulates cell growth and can initiate inflammation related carcinogenesis.
Description: The LTβR:LIGHT[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of LIGHT binding to LTβR . This kit comes in a convenient 96-well format, with biotin-labeled LIGHT, purified LTβR , streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled LIGHT by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LTβR is coated on a 96-well plate. Next, biotinylated LIGHT is incubated with LTβR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Biosafety Level: Not applicable (BSL-1)
References: Chang, J. H., et al. J Biomed Sci 2005, 12(2): 363-375.
Del Rio, M.L., et al. J. Leukoc. Biol. 2010, 87(2): 223-235
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