Application Note: Anti-Lysyl Oxidase antibody has been tested in WB, ICC/IF, IHC-F, IHC-P, IP. Expect a band approximately 31kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.
Immunohistochemistry Dilution: 1:100
IF Microscopy Dilution: User Optimized
Immunoprecipitation Dilution: User Optimized
Western Blot Dilution: 1:250-1:1000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Lysyl Oxidase Antibody was produced from whole rabbit serum prepared by repeated immunizations with the human protein Lysyl Oxidase at an internal amino acid sequence.
Physical State: Liquid
Purity and Specificity: Anti-Lysyl Oxidase Antibody is crude rabbit sera. A BLAST analysis was used to suggest cross-reactivity with Anti-Lysyl Oxidase from human, bovine, chimpanzee, mouse and rat 100% homology with the immunizing sequence. Predicted reactivity with chicken, dog, pig, and xenopus. Cross-reactivity with Anti-Lysyl Oxidase from other sources has not been determined.
Background: Anti-Lysyl Oxidase antibody detects human Lysyl Oxidase. Lysyl Oxidase (LOX) belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of ECM. Localized both in the cytosol and nucleus, it catalyzes the oxidation and deamination of epsilon-NH2 group of lysine and hydroxylysine residues to alpha-aminoadipic-delta-semialdehyde. This is the first step that initiates the covalent cross-linking of collagen and tropoelastin resulting in the formation of insoluble collagen and elastic fibers in the extracellular matrix. LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. Deficiency of LOX is seen in certain carcinomas and 3X-linked recessive human connective tissue disorders—Cutis Laxa. Anti-Lysyl Oxidase antibody is ideal for investigators involved in cytokines and growth factors.
Low Endotoxin: No
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