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Encompassing Amino Acids: 570-756(end)
Applications: Useful as an immunogen and for protein binding studies.
Assay Conditions: Enzymatic activity was confirmed by an AmplexRed/Peroxidase coupling method using 1,5-diaminopentane as a substrate. Purified LOXL4 (0.25-1 ng/µl) was incubated with 3 mM 1,5 diaminopentane, 0.4 mM AmplexRed and 0.2 unit/µl peroxidase in 100 µl of the assay buffer (1.2 M Urea, 50 mM borate pH 8.8) for 15 min at 30°C. Fluorescence intensity is measured every 7 min at 530ex/590em.
Description: Human Lysyl oxidase homolog 4 (LOXL4) also known as, Lysyl oxidase-related protein C (LOXC). GenBank Accession No. NM_032211, a.a. 570-756(end) with a C-terminal His-tag expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 9 mM Na2HPO4/KH2 PO4 pH 7.4, 124 mM NaCl, 2.4 mM KCl and 10% glycerol
Genbank: NM_032211
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His tag
Uniprot: Q96JB6
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Gorogh, T., et al., Int. J. Oncol. 2008; 33(5): 1091-1098.
2. Kim, D.J., et al., Biochem. Biophys. Res. Commun. 2008; 4: 521-527.
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