Background: Detection of mammaglobin in metastatic mamma carcinoma, differential diagnosis of metastases in sentinel lymph nodes Mammaglobin A is a breast gland associated glycoprotein, distantly related to the secretoglobin family including human Uteroglobin and Lipophilins. Unlike other secretoglobin family members Mammaglobin mRNA seems to be Mamma-specific (exception skin). Mammaglobin has been detected by immunohistochemical approach in intact breast tissue as well as in adenocarcinoma. It seems to be up-regulated in cancer tissues. The monoclonal antibody is suitable for the detection of occult mamma carcinoma cells in sentinel lymph nodes and in peripheral blood. The overall sensitivity of mammaglobin detection on paraffin sections of mamma carcinoma was reported to be >80% (Fanger et al. 2002; Han et al. 2003). 10 KDa Mammaglobin from human breast tissue, no cross-reactivity with mammaglobin B, lipophilin and uteroglobin.
Positive Control: Mamma carcinoma (normal mamma shows up lower expression of the protein), skin sweat glands
Immunogen: C-terminal 16 aa peptide sequence of human mammaglobin coupled to KLH as a carrier protein
Purification Method: Affinity purified antibody in stabilized phosphate buffer, containing 0.09 % sodium azide**. The volume is sufficient for app. 250 immunohistochemical tests (100 µl working solution per test). For dilution we recommend our antibody diluent Art. No. PU002.
Concentration: 50 µg/ml
References: 1. Watson MA, Dintzis S, Darrow CM, Voss LE, DiPersio J, Jensen R, Fleming TP. Mammaglobin expression in primary, metastatic, and occult breast cancer. Cancer Res. 1999 Jul 1;59(13):3028-31. 2. Fanger GR, Houghton RL, Retter MW, Hendrickson RC, Babcook J, Dillon DC,Durham MD, Reynolds LD, Johnson JC, Carter D, Fleming TP, Roche PC, Persing DH, Reed SG. Detection of mammaglobin in the sera of patients with breast cancer. Tumour Biol. 2002 Jul-Aug;23(4):212-21. 3. Han JH, Kang Y, Shin HC, Kim HS, Kang YM, Kim YB, Oh SY. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med. 2003 Oct;127(10):1330-4. 4. Gillanders WE, Mikhitarian K, Hebert R, et al. Molecular detection of micrometastatic breast cancer in histopathology-negative axillary lymph nodes correlates with traditional predictors of prognosis: an interim analysis of a prospective multi-institutional cohort study. Ann Surg. 2004 Jun;239(6):828-37; discussion 837-40. 5. Sjödin A., et al. Mammaglobin in normal human sweat glands and human sweat gland tumors. Journal of Investigative Dermatology (2003) 121(2): 428-429.
UniProt: Q13296
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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