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Encompassing Amino Acids: 2-360
Applications: Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using P38 peptide substrate (0.1 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)
Description: Mitogen-activated proteinkinase 14 (MAPK14) GenBankAccession No. NM_139012, a.a. 2-360(end), with N-terminal His-tag, MW 42.3kDa, expressed in a baculovirus-infectedSf9 cell expression system. This proteinis activated by in vitro phosphorylationand SEC.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% glycerol
Genbank: NM_139012
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoidfreeze-thaw cycles.
Tags: N-terminal His-tag
Uniprot: Q16539
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hatherley, D., et al., Mol. Cell, 2008,31:266-77
2. Baba, N., et al., J. Exp. Med., 2013,210(6):1251-63
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