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Encompassing Amino Acids: 2-359 (end)
Application: Useful for studying protein binding and screening small molecular inhibitors
Assay Conditions: The reactions were conducted at 30°C for 30 minutes in a 25 µl mixture containing 40 mM Tris-HCl, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, various amount of MAT2A enzyme, 0.1 mM L-Methionine, and 10 µM ATP. After enzymatic reactions, 25 µl of Kinase-Glo MAX reagent was added to each well and luminescence was measured using a BioTek Synergy™ 2 microplate reader.
Description: Human MAT2A, also known as methionine adenosyltransferase II alpha, MAT2, and adoMet synthase 2. GenBank Accession No. NM_005911, a.a. 2-359 (end) with an N-terminal His-tag, expressed in an E. coli cell expression system. MW = 45 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 167 mM imidazole
Genbank: NM_005911
Purity: ≥90%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: N-terminal His-Tag
Uniprot: P31153
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Latasa, M., et al. The FASEB Journal. 2001; 15(7): 1248-1250.2.Liu, Q., et al., Molecular Cancer Therapeutics. 2011; 10(6):1113-23.
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