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Applications: Useful for studying protein binding and screening small molecular inhibitors
Assay Conditions: The reactions were conducted at room temperature for 45 minutes in a 25 µl mixture containing 50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM KCl, 0.1 mg/ml BSA, various amount of MAT2a enzyme, 200 µM L-Methionine, and 200 µM ATP. After the enzymatic reactions, 50 µl of colorimetric detection reagent was added to each well and the reactions were incubated for another 15 min before the absorbance at 630 nm of each well was measured using a Tecan Infinite M1000 microplate reader.
Description: Human MAT2A, also known as methionine adenosyltransferase II alpha, MAT2, and adoMet synthase 2. GenBank Accession No. NM_005911, a.a. 2-395 (end) with an N-terminal His-tag, expressed in an E. coli cell expression system. MW = 45 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 20% glycerol, 250mM imidazole.
Genbank: NM_005911
Storage Stability: At least 6 months at -80°C.
Tags: N-Terminal His-Tag
Uniprot: P31153
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Latasa, M., et al., The FASEB Journal. 2001; 15(7): 1248-1250.2. Liu, Q., et al., Molecular Cancer Therapeutics. 2011; 10(6):1113-23
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