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Encompassing Amino Acids: 2-327
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay run according to MCL-1 TR-FRET Assay Kit (BPS Bioscience #79506). Enzyme mixture incubated for 1 hour at room temperature and TR-FRET measured at ex 340 nm / em 620 nm, 665 nm.
Description: Recombinant canine MCL1 (induced myeloid leukemia cell differentiation protein Mcl-1 homolog), encompassing amino acids 2-327. This construct contains an N-terminal His-tag (6xHis). The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT
Genbank: NP_001003016.2
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q8HYS5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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