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Encompassing Amino Acids: 2-498(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed according to MDM2 TR-FRET Assay Kit (BPS Bioscience #79773) with MDM2 titrated from 157.5 ng - 0 ng.
Description: Recombinant human MDM2 (Mouse double minute 2 homolog), full length encompassing amino acids 2-498 (end). In this construct the N-terminal end of MDM2 is fused with GST. A short peptide linker placed between GST and the protein includes a PreScission Protease Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT
Genbank: NM_002392
Purity: 0,52
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q00987
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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