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Encompassing Amino Acids: full length
Applications: Useful as a substrate of cRAF (RAF1) and other kinases.
Description: Human MEK1 (K97R) (GenBank Accession No. NM_002755), full length with lysine97 to arginine mutation and C-terminal His-tag, MW=44.3 kDa, expressed in E. coli. The K97R mutation prevents ATP binding, making the kinase inactive.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 10% glycerol, 200 mM imidazole, and 3 mM DTT.
Genbank: NM_002755
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: Q02750
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Smith,C.K. and Windsor,W.T. Biochemistry 46 (5), 1358-1367 (2007).
2. Burgermeister,E. et al. Mol. Cell. Biol. 27 (3), 803-817 (2007).
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