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Encompassing Amino Acids: 2-393 (end)
Applications: Useful as a substrate of cRAF (RAF1) and other kinases.
Description: Human MEK1, or mitogen-activated protein kinase kinase 1 (MAP2K1) (GenBank Accession No. NM_002755), full length a.a. 2 - 393 (end), with N-terminal GST-tag, MW = 69 kDa, expressed in baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Genbank: NM_002755
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q02750
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sugden, P.H. et al. Cell Signal. 1997 Aug, 9(5): 337-351.
2. Huynh, H. et al. BMC Gastroenterol. 2003 Aug 8, 3:19.
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