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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo Plus reagents (Promega Corporation, Madison, WI).
Description: Human MEKK2, also known as MAP3K2 and MEKK2B, GenBank Accession No. NM_006609, full length, N-terminal GST-tag, MW=96.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl , 20% glycerol and 3 mM DTT.
Genbank: NM_006609
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9Y2U5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Blank, J. L. et al. J. Biol. Chem. 271:5361-5368, 1996.
2. Hammaker, D. R. et al. J. Immun. 172:1612-1618, 2004.
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