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Encompassing Amino Acids: 1-340
Applications: Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 50 ng/ml BSA, 2 mM ATP, 5 µg of ZIPtide synthetic peptide substrate. Add [33P]-ATP and incubate at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Description: Recombinant human MELK,Genbank Accession #NM_014791, a.a. 1-30, with an N-terminal GST-tag, MW = 61kDa, expressed in Sf9 cells via abaculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutatione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Genbank: NM_014791
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q14680
Warnings: Avoidfreeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nakano, I., et al., J Cell Biol 2005;170:413-27.
2. Lin, ML., et al., Breast Cancer Res. 2007;9:R17.
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