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Encompassing Amino Acids: 2-76(end)
Applications: Useful as general ubiquitination inhibitor in the study of enzyme kinetics, screening inhibitors, and selectivity. Useful in studies that require reduced polyubiquitination chains.
Background: Covalent conjugation to ubiquitin (Ub) is one of the major post-translational modifications that regulates protein stability, function, and localization. Ubiquitination is the concerted action of three enzymes: a Ub-activating enzyme (E1), a Ub-conjugating enzyme (E2), and a Ub ligase (E3). The specificity and efficiency of ubiquitination are largely determined by the E3 enzyme, which directs the last step of the Ub-conjugating cascade by binding to both an E2ˆ¼Ub conjugate and a substrate protein. This step ensures the transfer of Ub from E2ˆ¼Ub to the substrate, leading to its mono- or poly-ubiquitination. Methylated ubiquitin is a derivative of ubiquitin where the lysine residues have undergone methylation. It can bind to substrates and block the formation of polyubiquitination chains.
Description: Methylated recombinant human ubiquitin, encompassing amino acids 2-76(end). This protein was affinity purified and methylated in vitro.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, and 1 mM TCEP
Genbank: NM_021009
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Warnings: Avoid freeze/thaw cycle
Biosafety Level: Not applicable (BSL-1)
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