Target: Methylglyoxal
Conjugate: RPE
Product Type: Monoclonal
Clone Number: 9F11
Immunogen: Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).
Purification: Protein G Purified
Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Scientific Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Methylglyoxal is an alpha-oxoaldehyde generated from lipid peroxidation that reacts with proteins to form adducts(1). It reacts with free amino acids and protein residues to generate advanced glycation endproducts (AGEs) and crosslinks DNA polymerase and substrate DNA to severely inhibit DNA replication (2).
References: 1. Negre-Salvayre et al. (2008) Br J Pharmacol. 153(1):6-20.2. Murata-Kamiya, N., Kamiya, H. (2001) Nucleic Acids Res. 29(16): 3433-3438.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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