Application Note: MIP-3ß Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MIP-3ß in immunological assays.
Concentration Value: 0.1 mg/mL
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: Biological Activity: Macrophage Inflammatory Protein 3ß is fully biologically active when compared to standard. The activity is calculated by the ability to chemoattract human T cells using a concentration of 7 - 40 ng/ml.Endotoxin Level: Measured by LAL is <0.01ng/µg or <0.1EU/µg.
Physical State: Lyophilized
Purity and Specificity: Recombinant protein corresponds to amino acids 22 to 98 of mature human MIP-3ß. Purity is greater than 98% as determined by reducing and non-reducing SDS-PAGE and by RP- HPLC, against known standard.
Background: The MIP-3ß (also known as SCYA19) member of the intercrine beta (chemokine CC) family may play a role not only in inflammatory and immunological responses, but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. It is secreted. It specifically binds to chemokine receptor CCR7. MIP3ß is expressed at high levels in the lymph nodes, thymus and appendix, and at intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. Recombinant Human MIP-3b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8809 Daltons. The recombinant protein shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes.
Low Endotoxin: Yes
Other: User Optimized
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