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Description: The
Mix & Go! E. coli Transformation Kit and
Mix & Go! E. coli Transformation Buffer Set are convenient methods for the preparation of competent
E. coli cells for simple and highly efficient DNA transformation. The
Mix & Go! method completely eliminates the requirement for heat shocking and related procedures. Instead,
Mix & Go! bacterial transformation can be performed by adding DNA to
Mix & Go! competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 10
8-10
9 transformants/µg plasmid DNA with most
E. coli strains. Uniquely formulated reagents make it easy to generate
Mix & Go! competent cells from current
E. coli strains that are available in the laboratory. Simply grow the
E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The
Mix & Go! E. coli Transformation Kit includes all buffers and ZymoBroth medium to generate 20 ml of
Mix & Go! competent cells. The
Mix & Go! E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of
Mix & Go! competent cells, and the medium (broth) is supplied by the user.
- Easy 3 Step Protocol: Produce reliable chemically competent E. coli in less then 45 minutes.
- Simple 20 sec Transformation: No heat shock! Just add DNA and spread.
- High Transformation Efficiencies: Achieve 108-109 transformants per µg of plasmid DNA.
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