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Encompassing Amino Acids: 1-446
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Varying concentrations of MLK2 were mixed with 5 µg of MBP protein substrate and 5 µl of 250 µM [33P]-ATP was added for a total volume of 25 µl. Mixture was incubated for 15 min at 30°C. Reaction was stopped by placing 20 µl of reaction onto phosphocellulose P81 paper. Paper was washed 3 times with 1% phosphoric acid solution for 10 min each and air dried. Radioactivity was measured from the P81 paper in the presence of scintillation fluid using a scintillation counter.
Description: Human MLK2, also known as Mixed Lineage Kinase 2, Mitogen-activated protein kinase kinase kinase 10, MAP3K10, and MST, GenBank Accession No. NM_002446, a.a. 1-446, with N-terminal GST-tag, MW = 76 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% Glycerol.
Genbank: NM_002446
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q02779
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Dorrow, D.S., et al., Europ. J. Biochem. 1995. 234:492-500.
2. Katoh, M., et al., Oncogene. 1995. 10:1447-1451.
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