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Encompassing Amino Acids: 1-488
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Varying concentrations of MLK3 were mixed with 5 µg of MBP protein substrate and 5 µl of 250 µM [33P]-ATP was added for a total volume of 25 µl. Mixture was incubated for 15 min at 30°C. Reaction was stopped by placing 20 µl of reaction onto phosphocellulose P81 paper. Paper was washed 3 times with 1% phosphoric acid solution for 10 min each and air-dried. Radioactivity was measured from the P81 paper in the presence of scintillation fluid using a scintillation counter.
Description: Human MLK3, also known as Mixed Lineage Kinase 3, Mitogen-activated protein kinase kinase kinase 11, MAP3K11, PTK1, SPRK, and MGC17114, GenBank Accession No. NM_002419, a.a. 1-488, with N-terminal GST-tag, MW = 83 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_002419
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q16584
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ing, Y.L. et al., Oncogene. 1994. 9:1745-1750.
2. Chadee, D.N. et al., Nature Cell Biol. 2004. 6:770-776.
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