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Encompassing Amino Acids: 1-557
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Varying concentrations of MLK4 were mixed with 5 µg of MBP protein substrate and 5 µl of 250 µM [33P]-ATP was added for a total volume of 25 µl. Mixture was incubated for 15 min at 30°C. Reaction was stopped by placing 20 µl of reaction onto phosphocellulose P81 paper. Paper was washed 3 times with 1% phosphoric acid solution for 10 min each and air dried. Radioactivity was measured from the P81 paper in the presence of scintillation fluid using a scintillation counter.
Description: Human MLK4, also known as Mixed Lineage Kinase 4, and KIAA1804, GenBank Accession No. NM_032435, a.a. 1-557, with N-terminal GST-tag, MW = 61 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_0324335
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q5TCX8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nagase, T., et al., DNA Res. 2001. 8:85-95.
2. Seit-Nebi, A., et al., Cell. Molec. Immun. 2012. 9:27-33.
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