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Encompassing Amino Acids: 2-end (deletion 165-205)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human MNK (T385D) (GenBank Accession No. NM_003684), a.a. 2-end with deletion a.a. 165-205 and threonine 385 to aspartic acid mutation, and N-terminal GST-tag, MW=74 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 3 mM DTT.
Genbank: NM_003684
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9BUB5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chang,S.H. et al., Am. J. Respir. Cell Mol. Biol. 35 (5), 528-539 (2006).
2. Gregory,S.G. et al., Nature 441 (7091), 315- 321 (2006).