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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: MNK2 (MAP kinase-interacting kinase 2) contains a conserved C-terminal ERK-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain. MNK2 binds tightly to the growth factor-regulated MAP kinases, ERK1 and ERK2. ERK and p38 phosphorylate MNK2, which stimulates its in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF-4E) (1). A yeast two-hybrid screen showed the Mnk2 protein interacted with the ligand-binding domain of estrogen receptor beta (ERbeta) (2).
Description: Recombinant human MNK2 (MAP kinase interacting serine/threonine kinase 2), also known as GPRK7 (G protein-coupled receptor kinase 7), GenBank Accession No. NM_017572, full length, with N-terminal GST tag, MW ~74 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_017572
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9HBH9
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Waskiewicz, A J. et al: Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. EMBO J. 1997 Apr 15;16(8):1909-20.
2. Slentz-Kesler, K. et al: Identification of the human Mnk2 gene (MKNK2) through protein interaction with estrogen receptor beta. Genomics. 2000 Oct 1;69(1):63-71.
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