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Applications: This kit is useful for screening for inhibitors of monkey PD-L1 binding to PD-1.
Description: Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and this pathway is exploited by both tumors and viruses. The Monkey PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling pathway in the animal model cynomolgus monkey (Macaca fascicularis). The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Storage Stability: Up to 6 months from date of receipt, when stored as recommended.
Supplied As: This kit comes in a convenient 96-well format, with purified monkey PD-L1, biotin-labeled monkey PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Onlamoon, N. et al. Immunol. 2008, 124.1: 277-293
Wang. C. et al. Cancer Immunol. Res. 2014, 2.9: 846-856
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