Clone: 3B3
Background: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Skeletal α-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development α-cardiac actin becomes the main isoform in the myocardium, α-skeletal actin might still make up for almost half of its total striated muscle actin content.
Source: 3B3 is a Mouse monoclonal IgG1 antibody derived by fusion of NS0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a peptide comprising the N-terminal nonapeptide of α-skeletal actin with an acetylated N-terminus (Ac-DEDETTALVC-COOH) coupled to keyhole limpet hemocyanin through the cysteine residue.
Specificity: Clone 3B3 is an antibody highly specific for α-skeletal actin, and does not cross react with other actin isoforms.
Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
References: 1. Vandekerckhove, J. and Weber, K. (1978). At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4), 783-802.2. Vandekerckhove, J., Bugaisky, G. and Buckingham, M. (1986). Simultaneous expression of skeletal muscle and heart actin proteins in various striated muscle tissues and cells. J Biol Chem. 261(4),1838-43.3. Suurmeijer, A.J., Clément, S., Francesconi, A., Bocchi, L., Angelini, A., Van Veldhuisen, D.J., Spagnoli, L.G., Gabbiani, G. and Orlandi, A. (2003). Alpha-actin isoform distribution in normal and failing Human heart: a morphological, morphometric, and biochemical study. J Pathol. 199(3), 387-97.4. Chaponnier, C. and Gabbiani, G. (2004). Pathological situations characterized by altered actin isoform expression. J Pathol. 204(4), 386-95.5. Driesen, R.B., Verheyen F.K., Debie, W., Blaauw, E., Babiker, F.A., Cornelussen, R.N., Ausma, J., Lenders, M.H., Borgers, M., Chaponnier, C. and Ramaekers, F.C. (2009). Re-expression of alpha skeletal actin as a marker for dedifferentiation in cardiac pathologies. J Cell Mol Med 13(5), 896-908.
UniProt: P68133
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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