Clone Number: LP18
Immunogen: A chemically synthesized phosphorylated peptide corresponding to residues around Ser473 of the human Akt molecule.
Concentration: Greater than or equal to 300 mg/L
Storage buffer: Tissue culture supernatant with 15mM sodium azide
Additional info: Akt-1, also referred to as protein kinase B (PKB) or Rac alpha is a member of the Akt serin/threonine protein kinase family. It plays an important role in many biological responses including metabolism, cell survival and growth by phosphorylation and inactivating several targets including GSK 3 beta, caspase 9, BAD and the forkhead transcription factor. Akt-Phos is not recommended for use with PBS, since the use of PBS-based wash buffers and possibly PBS-based antibody diluents gives increased background staining and decreased staining intensity.
References: Brazil D et al. Trends in Biochemical Sciences. 2004; 29(5):233–242/Nicholson K et al. Cellular Signalling. 2002; 14:381–395/Lawlor M et al. Journal of Cell Science. 2001; 114:2903–2910
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