Clone: RUU-WAC2A
Background: Annexin A5 (or annexin V) is a cellular protein of the annexin family. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin, and also to inhibit the activity of phospholipase A1. Annexin A5 forms a shield around negatively-charged phospholipid molecules, which blocks the entry of phospholipids into coagulation (clotting) reactions. Antibodies directed anti annexin A5 are the cause of the antiphospholipid syndrom in which the formation of the shield is disrupted by antibodies. Without the shield, there is an increased quantity of phospholipid molecules on cell membranes, speeding up coagulation reactions and causing the blood-clotting characteristic of the antiphospholipid antibody syndrome. Annexin A5 is used as a probe in the annexin A5 affinity assay to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis aswell as other forms of cell death (1,2). Platelets also expose phosphatidyl-serine on their surface when activated, which serves as binding site for various coagulation factors. Annexin A5 has been shown to interact with kinase insert domain receptor (3) and integrin beta 5 (4). RUU-WAC2a is a monoclonal that specifically reacts with annexin A5. This monoclonal antibody is directed to the phospholipid binding domain of annexin A5 and as such it inhibits annexin A5 binding to anionic phospholipid membranes for more than 95%. RUU-WAC 2A does not remove annexin A5 bound to phospholipid surfaces indiCating that the epitope is not accessible after interaction with annexin A5.
Source: RUU-WAC2a is a Mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with recombinant annexin A5.
Specificity: RUU-WAC2A reacts anti Human recombinant and enDogenous annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognise Monkey and Rat Annexin V in ELISA.
Formulation: Each vial contains 100 ul of 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
References: 1. Koopman, G., Reutelingsperger, C.P., Kuijten, G.A.M. et al. (1994). Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84, 1415-20. 2. Vermes, I., Haanen, C., Steffens-Nakken, H. and Reutelingsperger, C. (1995). A novel assay for apoptosis-flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184, 39. 3. Wen, Y., Edelman J.L., Kang, T. and Sachs, G. (1999). Lipocortin V may function as a signaling protein for vascular endothelial growth factor receptor-2/Flk-1. Biochem. Biophys. Res. Commun. 258, 713–21. 4. Cardó-Vila, M., Arap, W. and Pasqualini, R. (2003). Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annex V. Mol. Cell 31, 1151-62. 5. Van Heerde, W.L. (1994). Thesis; localization, plasma levels and anticoagulant activity. 6. Van Heerde, W.L., Reutelingsperger, C.P., Maassen, C., Lux, P., Derksen, R.H. and De Groot, P.G. (2003). The presence of antiphospholipid antibodies is not related to increased levels of annexin A5 in plasma. Jn Thromb Haemost. 3, 532-536. 7. Van Heerde, W.L., Lap, P., Schoormans, S., De Groot, P.G., Reutelingsperger, C.P.M. and Vroom, T.M. (2004). Localization of annexin A5 in Human tissues. Annexins 1, 37-43.
UniProt: P08758
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
English
Deutsch
