Clone Number: HE-195
Immunogen: Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Concentration: 1 mg/ml
Format: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Storage buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Additional info: Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.SpecificityThe mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.Application detailsFlow cytometry: Recommended dilution: 1-4 μg/ml.
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