Mouse anti-CD45, clone Bra55 (Monoclonal)

Clone Number: Bra55

Immunogen: cells of the non-T, non-B acute lymphoblastic leukemia cell line REH 6.

Concentration: 100 ug/ml

Storage buffer: PBS with 0.02% sodium azide

Additional info: CD45 glycoprotein have various molecular weight on various cell types: B-cells 240 kDa, thymocytes 180 kDa, T-cells multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Isoforms are produced by alternative splicing of domains 4, 5 and 6. Various isoform are expressed differently on different lymphocytes. All hematopoietic cells express CD45 proteins except erythrocytes. Relevant epitopes are termed CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45R or CD45R0 (exon 4-6 spliced out).

References: Chorváth et al., Neoplasma 34(6), 685-692, (1987)/Chorváth et al., Neoplasma 35(5), 495-501, (1988/Chorváth et al. Leukocyte Typing IV, pp. 634-637, (1989)

• SKU: SANMON9943
• Manufacturer: Sanbio / Monosan
• Manufacturer SKU: MON9943
• Green Labware: No
• Package Unit: 100 µg
• Quantity Unit: STK
• Reactivity: Human
• Clonality: Monoclonal
• Application: Immunofluorescence, Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Western Blotting, ELISA, Flow Cytometry
• Isotype: IgG1-k
• Host: Mouse