Clone Number: DCS-6
Immunogen: recombinant human cyclin D1 (amino acids 1-295)
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.SpecificityThe antibody VIK-10 reacts with cytokeratin 10 (56.5 kDa; intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.Application detailsImmunohistochemistry: Recommended dilution: 5-10 μg/ml.
Flow cytometry: Recommended dilution: 3-12 µg/ml. Intracellular staining.
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