Clone: M6
Background: Diacylglycerol kinase alpha is an enzyme that in humans is encoded by the DGKA gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Tissue specificity: lymphocytes and oligodendroglial cells. Diacylglycerol kinases are involved in cell signaling, either as regulators of diacylglycerol levels or as intracellular signal-generating enzymes.
Source: M6 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with an Escherichia Coli cell-expressed, affinity-purified glutathione S-transferase fusion protein of a C-terminal portion (part of the catalytic domain) of rat DGKa.
Specificity: M6 is directed anti the C-terminal portion (part of the catalytic domain) of rat DGKa.
Formulation: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
References: 1. Brahim Houssa, Dick Schaap, Jose´ van der Wal, Kaoru Gotoi, Hisatake Kondoi, Akio Yamakawa, Masao Shibata, Tadaomi Takenawa, and Wim J. van Blitterswijk (1997) Cloning of a novel human diacylglycerol kinase (DGKtheta) containing three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. THE JOURNAL OF BIOLOGICAL CHEMISTRY 272, 10422–10428. 2.Cutrupi S, Baldanzi G, Gramaglia D, Maffè A, Schaap D, Giraudo E, van Blitterswijk W, Bussolino F, Comoglio PM, Graziani A. (2000) Src-mediated activation of alpha-diacylglycerol kinase is required for hepatocyte growth factor-induced cell motility. EMBO J.19:4614-4622.
UniProt: P23743
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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