Clone: RB1.
Background: DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent.
Purification Method: Protein A/G Chromatography.
Source: Hybridoma obtained after immunization of mice with recombinant DNA Adenine Methylase protein.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Pogolotti, AL Jr., et al. 'On the mechanism of DNA-adenine methylase.' J Biol Chem, 1988, 263, 7461-7464.
2. Heithoff, D.M., et al. ‘An essential role for DNA adenine methylation in bacterial virulence.’ Science, 284, 967-970.
UniProt: P0AEE8.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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