Clone: F56-BA1.2.3.
Background: Green Fluorescent Protein (GFP) from Jellyfish (Aequorea Victoria) is a 27kDa protein that provides strong visible fluorescence. GFP can be used as a reporter of gene expression, a tracer of cell lineage, and a fusion tag to monitor protein localization from living cells. It can also be used to access protein-protein interactions in yeast two hybrid systems and measure distances between proteins in fluorescence energy transfer (FRET).
Positive Control: Purified Green Fluorescent Protein.
Purification Method: Protein A/G Chromatography.
Source: Hybridoma produced by the fusion of splenocytes of mice immunized with full-length green fluorescent protein and mouse myeloma cells.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Prasher,D.C et.al. 'Primary structure of the Aequorea victoria green-fluorescentprotein.' Gene 111 (2), 229-233 (1992)
2. Inouye,S. and Tsuji,F.I. 'Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein' FEBS Lett. 341 (2-3), 277-280 (1994)
3. Cody,C.Wet.al. 'Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein' Biochemistry 32 (5), 1212-1218 (1993.
UniProt: P42212.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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