Clone: 2C5.1.
Background: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only.
Purification Method: Protein A/G Chromatography.
Source: Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells.
References: 1. Zentgraf, H., et al. Detection of histidine-tagged fusion proteins by using a high-specific mouse monoclonal anti-histidine tag antibody. Nucleic Acids Res. 1995, 23, 3347-3348
2. Sisk, W.P., et al. High-level expression and purification of secreted forms of herpes simplex virus type 1 glycoprotein gD synthesized by baculovirus-infected insect cells. J. Virol. 1994, 68, 766-775
3. Hosfield, T. and Lu, Q. S. pombe expression vector with 6x(His) tag for protein purification and potential for ligation-independent cloning. Biotechniques 1999, 27, 50-60.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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