Clone Number: MEM-61
Immunogen: Pre-B cell line NALM-6.
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.SpecificityThe mouse monoclonal antibody VIM15 recognizes an extracellular epitope of CD92, a 70 kDa transmembrane glycoprotein expressed mainly on human peripheral blood monocytes and neutrophils, and several myeloid and T-cell lines.Application detailsFlow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 2-4 μg/ml, non-reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 20 μg/ml, positive tissue: prostate.
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