Clone Number: CH2
Conjugate: HRP
Immunogen: Purified human IgM.
Concentration: 1 mg/ml
Format: Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
Storage buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal
Additional info: Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.SpecificityApplication details Western blotting: Recommended dilution: 1 μg/ml.
Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular and intracellular staining.