Clone: NI 285, NI 250
Background: The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains, as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining and ELISA and immunoblotting using an indirect technique with monoclonal anti FITC labelled with peroxidase. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Source: Highly purified Bence Jones kappa proteins isolated from pooled human urine.
Specificity: Fluorescein isothiocyanate-conjugated purified monoclonal Mouse antibody to Human Ig kappa chain, free and bound
Formulation: Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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