Clone: NI 315 (HP 6206)
Label: FITC
Background: The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50; in ELISA from 1:200 upwards; in Western blotting from 1:400 upwards.
Source: Highly purified monoclonal IgG4 isolated from human serum.
Specificity: Fluorescein isothiocyanate-conjugated purified monoclonal Mouse antibody to Human IgG4, subclass specific.In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the MAHu/IgG4/FITC. As a detection antibody an HRP-conjugated mouse anti-FITC was used. Reaction with human IgG1, IgG2 and IgG3 was minimal.
Formulation: Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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